DNA

Part:BBa_K4765131

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-08)


ribozyme connected: intimin-Nb1 + mScarlet contributed by Fudan iGEM 2023

Usage and Biology

This composite part is the version of BBa_K4765102 with mScarlet added. We use this part to observe the biofilm formation process.

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(2) indicate the successful construction of intimin-Nb1 and intimin-Nb1 + mScarlet.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 3000
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305
[edit]
Categories
//collections/biofilm
Parameters
proteins