Device

Part:BBa_K4720104

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


J23100+RBS+FleQ+TT+Pcl+Ppel+RBS+Lux

BBa_J23100 + RBS (BBa_J34801) FleQ (BBa_K4720000) + Double Terminator (BBa_B0015) + Pcl (BBa_R0051) + Ppel (BBa_K4242001) + RBS (BBa_J34801) + Lux (BBa_K4720105)

This is a theoretical part we planned on making but did not obtain due to limited time. It is a combination of the cyclic-di-GMP sensor (BBa_K4720017) with the lux reporter (BBa_K4720105). It is an alternative to BBa_K4720021 with the advantage that no excitation is required. The disadvantage is that the response is expected to less direct.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 7183
    Illegal BamHI site found at 233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4840
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3220
    Illegal SapI site found at 5359


[edit]
Categories
Parameters
None