Composite

Part:BBa_K4720023

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-12)


GFP+T+Pcl+RBS+RFP+TT

GFP (BBa_E0040) + Terminator (BBa_B1006) + RBS (BBa_B0034) + RFP (BBa_E1010) + Double Terminator (BBa_B0015)

This part is an improvement on the part BBa_K4720015. The repeating sequence of the Double Terminator was replaced by Terminator BBa_B1006. This is supposed to avoid recombination.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1348
    Illegal AgeI site found at 1460
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None