Coding

Part:BBa_K4720000

Designed by: Jelle de Boer   Group: iGEM23_Groningen   (2023-10-10)


Codon Optimized FleQ

This part is an improvement on the part BBa_K4242000 by CUG China 2022. The part has been improved by codon optimization of FleQ. And the removal of three SapI restriction sites, making the part RFC [1000] compatible.

FleQ is the repressor protein of the Ppel promoter (BBa_K4242001). It can bind Cyclic-di-GMP resulting in a conformational change that activates the Ppel promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 172
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None