Part:BBa_K4701224

1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase (tphB)

1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate (DCD) dehydrogenase (UniProt: Q3C1D3) encoded by the tphB_II gene from Comamonas sp. E6 is responsible for catalyzing a reaction from DCD to protocatechuate (PCA) [1]. This reaction is the second one in a common TPA assimilation pathway [2]. This part contains the coding sequence of tphB_II optimized for expression in Pseudomonas putida, and more specifically, in a synthetic operon to be expressed along with 4 other proteins.

For more details, see the design page of BBa_K4701306

References

[1] Sasoh, M. et al. (2006) Characterization of the Terephthalate Degradation Genes of Comamonas sp. Strain E6. Applied and Environmental Microbiology. 72(3), 1825–1832. https://doi.org/10.1128/AEM.72.3.1825-1832.2006

[2] Salvador, M. et al. (2019) Microbial Genes for a Circular and Sustainable Bio-PET Economy. Genes. 10(5), 373. https://doi.org/10.3390/genes10050373

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]