Composite

Part:BBa_K4320027

Designed by: Angela Gao   Group: iGEM22_MIT   (2022-10-11)


pVeg-spoVG-acsAB-T001

Gene expression cassette consisting of acsAB under the Pveg promoter. Basic parts Pveg, SpoVG, acsAB, and T001 were cloned into the pL1f1 plasmid backbone for transformation into E. coli. Integrating this genetic circuit into E. coli would allow for us to grow our patch from bacterial cellulose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1887
    Illegal BglII site found at 2601
    Illegal BglII site found at 4524
    Illegal BamHI site found at 897
    Illegal BamHI site found at 3891
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1873
    Illegal AgeI site found at 1950
    Illegal AgeI site found at 2506
    Illegal AgeI site found at 3307
    Illegal AgeI site found at 3399
    Illegal AgeI site found at 3505
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None