Composite

Part:BBa_K4320026

Designed by: Angela Gao   Group: iGEM22_MIT   (2022-10-11)


CCP1_mod-mRuby2-cyc1

Gene expression cassette consisting of mRuby2 under the CCP1_mod promoter. Basic parts CCP1_mod, mRuby2, and cyc1 were cloned into the pYTK_096 backbone to allow for integration into the URA3 locus in S. cerevisiae. We planned to use this part to characterize the activity of the CCP1_mod promoter and measure resulting mRuby2 fluorescence at various time points after adding hydrogen peroxide. We also planned to use this experiment to compare the activity of our modified CCP1 (with no internal BsaI sites) against the natural CCP1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
    Illegal NgoMIV site found at 613
    Illegal NgoMIV site found at 623
  • 1000
    COMPATIBLE WITH RFC[1000]


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