Part:BBa_K4320022

CCP1-GSH1-cyc1

Gene expression cassette consisting of GSH1 under the CCP1 promoter. Basic parts CCP1, GSH1, and cyc1 were cloned into the pYTK_097 backbone to allow for integration into the LEU2 locus in S. cerevisiae. Integrating this genetic circuit into yeast would allow for our patch to overexpress the Gsh1 enzyme to allow for upregulated glutathione synthesis only under conditions of oxidative stress that would lead to ulcer formation. We did not want our patch to synthesize excess glutathione unnecessarily. We decided to express both ADP1 and GSH1 under the control of the oxidative stress inducible promoter CCP1 in order to somewhat balance increased synthesis of glutathione with increased export to prevent potential cellular toxicity.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2170
  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2170
  Illegal NheI site found at 1641
  Illegal NheI site found at 1680
  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2170
  Illegal BglII site found at 453
  Illegal BamHI site found at 1275
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2170
  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2170
  Illegal SpeI site found at 290
  Illegal SpeI site found at 364
  Illegal NgoMIV site found at 613
  Illegal NgoMIV site found at 623
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 158