Composite

Part:BBa_K4320021

Designed by: Angela Gao   Group: iGEM22_MIT   (2022-10-11)


CCP1-ADP1-cyc1

Gene expression cassette consisting of ADP1 under the CCP1 promoter. Basic parts CCP1, ADP1, and cyc1 were cloned into the pYTK_096 backbone to allow for integration into the URA3 locus in S. cerevisiae. Integrating this genetic circuit into yeast would allow for our patch to overexpress the Adp1 glutathione export protein and export glutathione into the gums only under conditions of oxidative stress that would lead to ulcer formation. We did not want our patch to secrete excess glutathione unnecessarily. We decided to express both ADP1 and GSH1 under the control of the oxidative stress inducible promoter CCP1 in order to somewhat balance increased synthesis of glutathione with increased export to prevent potential cellular toxicity.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3102
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3102
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3102
    Illegal BglII site found at 453
    Illegal BglII site found at 2863
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3102
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3102
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
    Illegal NgoMIV site found at 613
    Illegal NgoMIV site found at 623
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 158


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