Plasmid_Backbone

Part:BBa_K4320009

Designed by: MIT iGEM 2022   Group: iGEM22_MIT   (2022-10-11)


pYTK_098

pYTK_098 is a level 1 or 2 pre-assembled S. cerevisiae URA3 plasmid that we assembled using the Duber YTK MoClo Kit. It contains a GFP dropout sequence, is compatible with BsaI (level 1) and BsmBI (level 2) and has kanamycin resistance in E. coli and LEU selection for integration into S. cerevisiae. It was assembled from the parts ConLS, GFP dropout, ConR1, URA3, 2micron, KanR-ColE1 from the Duber YTK MoClo Kit.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
    Illegal NotI site found at 3466
    Illegal NotI site found at 5229
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 10
    Illegal SpeI site found at 1379
    Illegal PstI site found at 1388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1214
    Illegal BsaI.rc site found at 196
    Illegal SapI.rc site found at 380
    Illegal SapI.rc site found at 2090


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