Regulatory

Part:BBa_K4320000

Designed by: MIT iGEM 2022   Group: iGEM22_MIT   (2022-10-11)


CCP1

CCP1 encodes mitochondrial cytochrome-c peroxidase, an enzyme that degrades reactive oxygen species in the mitochondria. In cells, it acts as a hydrogen peroxide sensor in mitochondria to produce a cellular response to oxidative stress (SGD:S000001774). We chose to utilize this inducible promoter for our system so we could overexpress GSH1 and ADP1 to increase glutathione synthesis and export only in response to elevated levels of oxidative stress in the environment. This conditional gene expression would increase the metabolic fitness of the yeast in our patch.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 290
    Illegal SpeI site found at 364
    Illegal NgoMIV site found at 613
    Illegal NgoMIV site found at 623
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 158


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