Plasmid

Part:BBa_K4113028

Designed by: Wenhui Ren   Group: iGEM22_FAFU-China   (2022-10-10)


pCAMBIA1301SN plasmid

Using pCAMBIA1301 plasmid as template, a 538bp CaMV35S promoter fragment and a 260bp NOS terminator fragment were obtained after subcloning. The TA clone was sent to sequencing. The clone with correct sequence alignment was sequentially ligated to the polyclonal site of pCAMBIA1301 vector after double digestion for two times. The recombinant plasmid was constructed and sequenced. Sequencing results showed that the size and position of the inserted fragment were correct, indicating that the intermediate expression vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2556
    Illegal NheI site found at 6000
    Illegal NotI site found at 5407
    Illegal NotI site found at 6697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 11588
    Illegal XhoI site found at 9443
    Illegal XhoI site found at 10537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3320
    Illegal NgoMIV site found at 3494
    Illegal NgoMIV site found at 3544
    Illegal NgoMIV site found at 3548
    Illegal NgoMIV site found at 3575
    Illegal NgoMIV site found at 4074
    Illegal NgoMIV site found at 4432
    Illegal AgeI site found at 6494
    Illegal AgeI site found at 10164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6397
    Illegal SapI site found at 7088
    Illegal SapI.rc site found at 6625


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