Coding

Part:BBa_K4113009

Designed by: Wenhui Ren   Group: iGEM22_FAFU-China   (2022-10-06)


DlGAD2

Glutamic acid decarboxylase (GAD) is a key rate-limiting enzyme in the synthesis pathway of the inhibitory neurotransmitter γ-aminobutyric acid (GABA), which plays an important function in plant cellular adversity defense, nitrogen storage, hormone synthesis, and growth and development. When plants are subjected to external environmental stimuli (e.g. mechanical damage, drought, frost, etc.), intracellular H+ or Ca2+ levels increase, which in turn leads to the activation of GAD and a significant increase in GABA content


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1236
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 59
    Illegal BamHI site found at 372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1408


[edit]
Categories
Parameters
None