Composite

Part:BBa_K4103026

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-20)


Secretion Tag library 5

Library plasmid containing Bacillus subtilis secretion tags and associated ribosome binding sites, defined as RBSLoc parts in the ProClo/expanded FreeGenes MoClo assembly standard. Digestion with BsaI will free all the parts to participate in a Moclo assembly reaction, the transformants of which can then be screened for secretion efficiency. PmeI could be added to the digestion reaction to prevent multiple tags from assembling in tandem into the finished constructs. Plasmid 1 of 6 in the library.

Available at https://stanford.freegenes.org/products/b-subtilis-protein-secretion-toolkit?_pos=1&_psq=se&_ss=e&_v=1.0#description

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3472
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 3472
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3472
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3472
    Illegal NgoMIV site found at 2727
    Illegal NgoMIV site found at 3670
    Illegal AgeI site found at 1181
    Illegal AgeI site found at 4701
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2
    Illegal BsaI site found at 181
    Illegal BsaI site found at 372
    Illegal BsaI site found at 545
    Illegal BsaI site found at 733
    Illegal BsaI site found at 987
    Illegal BsaI site found at 1184
    Illegal BsaI.rc site found at 107
    Illegal BsaI.rc site found at 298
    Illegal BsaI.rc site found at 471
    Illegal BsaI.rc site found at 659
    Illegal BsaI.rc site found at 913
    Illegal BsaI.rc site found at 1110
    Illegal BsaI.rc site found at 1322


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