Composite

Part:BBa_K4103024

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-20)


Secretion Tag library 3

Library plasmid containing Bacillus subtilis secretion tags and associated ribosome binding sites, defined as RBSLoc parts in the ProClo/expanded FreeGenes MoClo assembly standard. Digestion with BsaI will free all the parts to participate in a Moclo assembly reaction, the transformants of which can then be screened for secretion efficiency. PmeI could be added to the digestion reaction to prevent multiple tags from assembling in tandem into the finished constructs. Plasmid 1 of 6 in the library.

Available at https://stanford.freegenes.org/products/b-subtilis-protein-secretion-toolkit?_pos=1&_psq=se&_ss=e&_v=1.0#description

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1746
    Illegal PstI site found at 4265
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1746
    Illegal PstI site found at 4265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1746
    Illegal PstI site found at 4265
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1746
    Illegal PstI site found at 4265
    Illegal NgoMIV site found at 2514
    Illegal AgeI site found at 786
    Illegal AgeI site found at 2049
    Illegal AgeI site found at 4195
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 39
    Illegal BsaI site found at 237
    Illegal BsaI site found at 455
    Illegal BsaI site found at 640
    Illegal BsaI site found at 837
    Illegal BsaI site found at 1031
    Illegal BsaI site found at 1246
    Illegal BsaI.rc site found at 162
    Illegal BsaI.rc site found at 381
    Illegal BsaI.rc site found at 566
    Illegal BsaI.rc site found at 763
    Illegal BsaI.rc site found at 957
    Illegal BsaI.rc site found at 1172
    Illegal BsaI.rc site found at 1372


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