Composite

Part:BBa_K4103022

Designed by: Almiro Pires da Silva Neto   Group: iGEM21_Open_Science_Global   (2021-10-20)


Secretion Tag library 1

Library plasmid containing Bacillus subtilis secretion tags and associated ribosome binding sites, defined as RBSLoc parts in the ProClo/expanded FreeGenes MoClo assembly standard. Digestion with BsaI will free all the parts to participate in a Moclo assembly reaction, the transformants of which can then be screened for secretion efficiency. PmeI could be added to the digestion reaction to prevent multiple tags from assembling in tandem into the finished constructs. Plasmid 1 of 6 in the library.

Available at https://stanford.freegenes.org/products/b-subtilis-protein-secretion-toolkit?_pos=1&_psq=se&_ss=e&_v=1.0#description

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1878
    Illegal PstI site found at 2242
    Illegal PstI site found at 3708
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1533
    Illegal BsaI site found at 1730
    Illegal BsaI site found at 1972
    Illegal BsaI site found at 2172
    Illegal BsaI site found at 2375
    Illegal BsaI site found at 2596
    Illegal BsaI site found at 2793
    Illegal BsaI.rc site found at 1656
    Illegal BsaI.rc site found at 1898
    Illegal BsaI.rc site found at 2098
    Illegal BsaI.rc site found at 2301
    Illegal BsaI.rc site found at 2522
    Illegal BsaI.rc site found at 2719
    Illegal BsaI.rc site found at 2925


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