Plasmid_Backbone

Part:BBa_K4096000

Designed by: Ke ZHU   Group: iGEM21_Shanghai_Metro_HS   (2021-10-12)


pET-25b-vector

pET-25b-vector

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 197
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BglII site found at 446
    Illegal BamHI site found at 252
    Illegal XhoI site found at 212
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 285
    Illegal NgoMIV site found at 478
    Illegal NgoMIV site found at 2066
    Illegal NgoMIV site found at 2226
    Illegal NgoMIV site found at 5406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None