B domain of Staphylococcal protein A fused to amilGFP chromoprotein
B domain of Staphylococcal protein A fused to amilGFP chromoprotein.
The part contains a strong constitutive promoter, RBS, and the amilGFP-protein A fusion protein.
Usage and Biology
Protein A is a well-known protein from Staphylococcus aureus, abbreviated as SpA. It contains five highly homologous Ig-binding domains in tandem: domains E, D, A, B, and C. SpA acts as a virulence factor thanks to these immunoglobulin-binding domains, which bind to the constant fragments (Fc) of IgG antibodies facilitating immune evasion. 
Protein A is commonly used in molecular biology, i.e. for Tandem affinity purification, or in biotechnological applications, i.e. Affibodies. It also has a role in therapy, since SpA-based immunoadsorption has been proposed as a treatment for patients suffering from autoimmune diseases as well as to reverse severe antibody-mediated rejection in organ transplantations. 
AmilGFP is a yellow chromoprotein commonly used reporter. Its bright yellow colour can be observed by the naked eye, but it can also be quantified using spectrophotometry (absorption maximum at 502 nm and emission maximum at 512 nm). 
Since protein A is a promising target for S. aureus detection systems and anti-S.aureus therapy, our amilGFP - protein A fusion protein will be useful for iGEM teams working with S. aureus-related projects.
 J. Ren, ... L.-Y. Jia. Comprehensive Biotechnology (Second Edition), 2011
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21Illegal BglII site found at 860
Illegal BamHI site found at 778
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC