Coding

Part:BBa_K407015

Designed by: Syed Habib Tahir Bukhari, Ashwini Rahul Akkineni, Adithya Ananth, Victor Gordeev, Svea Grieb, Sarah Mansour, Mareike Roth, Charanya Sampathkumar, Lucas Schirmer, Jonathan Tam   Group: iGEM10_BIOTEC_Dresden   (2010-10-26)


MBP + TEV cleavage site

The maltose binding protein (MBP) can be used as purification tag. After purification the tag can be removed using a protease specifically cutting at the TEV site (Tobacco Etch Virus site).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 365
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 63


[edit]
Categories
Parameters
None