Composite

Part:BBa_K4060517:Design

Designed by: Cheng-Ju Lu   Group: iGEM21_NYCU-Taipei   (2021-10-21)


BBa_K4060503 + BBa_K4060511


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 657
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 657
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 657
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 657
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 657
    Illegal SpeI site found at 693
    Illegal PstI site found at 1050
    Illegal NgoMIV site found at 354
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construction of this part is according to igem assembly protocol.


Source

The LexA gene is cloned from E.coli K12 MG1655 genomic DNA. The QPAS1 gene is cloned from Addgene plasmid pKA-207I10. The remaining parts are from 2019 igem kits.

References