Part:BBa_K404301
GSAT-Linker
| [GSAT-Linker | |
|---|---|
| BioBrick Nr. | BBa_K404301 |
| RFC standard | RFC 25 |
| Requirement | pSB1C3 |
| Source | pAAV_MCS: provided by Stratagene |
| Submitted by | [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010] |
Amino acid sequende:
GGSAGGSGSGSSGGSSGASGTGTAGGTGSGSGTGSG
This part is a peptide linker, it can be used to connect two protein parts / domains and add additional space between them. This can be necessary to avoid interactions between the different parts when fusion proteins are created. The GSAT linker was created to connect our protein domains to a fusion construct.
Usage and Biology
Peptide linkers are used to connect two peptides. In nature, many of them connect individual domains of a protein. They are often flexible and vary in length. Especially for the creation of artificial fusion proteins, it is important for this connection that the linker itself has no influence on the connected peptides. Therefore the sequence of the linker is designed with amino acids which do not interact with amino acids in their environment. The amino acids glycine and serine are zwitterionic and hydrophile - these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. If the linker is too short, there might be a sterical interference between the parts and if it is too long, it can cause instability of the construct. It is also important that the linker has a certain flexibility although too much flexibility may interfere with the function of the linked peptides.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |