- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 387
For designing the BioBrick part, the iGEM team Freiburg used primers in the RFC10 standard and performed a PCR. The PCR product was digested using XbaI and PstI followed by ligation into the iGEM standard plasmid pSB1C3.
The plasmid was PCR amplified using the vector pAAV_MCS as DNA template.
Danckwardt, S., Hentze, M. W., & Kulozik, A. E. (2008). 3' end mRNA processing: molecular mechanisms and implications for health and disease. The EMBO journal, 27(3), 482-98. doi: 10.1038/sj.emboj.7601932.
Millevoi, S., Loulergue, C., Dettwiler, S., Karaa, S. Z., Keller, W., Antoniou, M., et al. (2006). An interaction between U2AF 65 and CF I(m) links the splicing and 3' end processing machineries. The EMBO journal, 25(20), 4854-64. doi: 10.1038/sj.emboj.7601331.