Synthetic Receptor CARζ
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal XhoI site found at 344
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 952
Illegal NgoMIV site found at 1288
Illegal NgoMIV site found at 1825
Illegal NgoMIV site found at 1891
Illegal NgoMIV site found at 2050
Illegal AgeI site found at 440
- 1000Illegal BsaI.rc site found at 1168
Illegal BsaI.rc site found at 1432
Illegal SapI.rc site found at 1989
The structure and function of CAR ζ
CAR ζ is another CAR aimed to increase the phagocytosis of the macrophages. And the structure is also similar to the other three CARs, with the difference in the intracellular domain which is replaced by CD3ζ(BBa_K4040003) subunit of the T cell receptor.
The mechanism of CAR ζ
In T cells, phosphorylated ITAMs in CD3z bind to tandem SH2 domains (tSH2) in the kinase ZAP70. Zap70 is not expressed in macrophages, but Syk, a phagocytic signaling effector and tSH2 domain containing protein, is expressed at high levels (Andreu et al., 2017). Previous work suggested that Syk kinase can also bind to the CD3z ITAMs (Bu et al., 1995), indicating that the CAR-T may promote engulfment through a similar mechanism as CAR-PFcRV . To quantitatively compare the interaction between SyktSH2 and CD3z or FcRV in a membrane proximal system recapitulating physiological geometry, previous study used liposome-based assay (Figure 1 [Hui and Vale, 2014]). In this system, His10-CD3z and His10-Lck (the kinase that phosphorylates CD3z) are bound to a liposome via NiNTA-lipids and the binding of labeled tandem SH2 domains to phosphorylated CD3z was measured using fluorescence quenching. Their results show that Syk-tSH2 binds the CD3z and FcRV with comparable affinity (~15 nM and ~30 nM respectively, Figure 1). Collectively, these results demonstrate that the TCR CD3z chain can promote phagocytosis in a CAR-P, likely through the recruitment of Syk kinase.
Results of our project
It can be found in BBa_K4040018
Morrissey MA, Williamson AP, Steinbach AM, Roberts EW, Kern N, Headley MB, Vale RD. Chimeric antigen receptors that trigger phagocytosis. Elife. 2018 Jun 4;7:e36688. doi: 10.7554/eLife.36688. PMID: 29862966; PMCID: PMC6008046.