Part:BBa_K4010011
Constitutively Expressed QseC
Figure 1: Sequence overview of BBa K4010011.
The two-component Quorum Sensing E. coli regulator B and C system (QseBC) is responsible for inducing flagella expression and therefore bacteria motility (Clark et al., 2006). QseC is a histidine sensor kinase that acts as a membrane-bound adrenergic receptor, responding to both microbiota-derived autoinducer-3 and the host hormonal stress signals epinephrine and norepinephrine (Clark et al., 2006; Rooks et al., 2017; Sperandio et al., 2002). Upon activation, QseC autophosphorylates and transfers a phosphate to QseB, which is the response regulator in this signaling cascade (Clark et al., 2006; Rooks et al., 2017). Phosphorylated QseB directly binds to both the high and low affinity sites of the flhDC promoter region, leading to transcription of downstream flagellum regulator genes (Clark & Sperandio, 2005). Since both sufficient quantity and phosphorylation of QseB are required for full activation of flagella regulon, the bacteria ensures the presence of survivable conditions with ample resources in host before flhDC transcription (Hughes et al., 2009).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1003
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Attribution Note: Figure created in Biorender.
Clarke, M. B., & Sperandio, V. (2005). Transcriptional regulation of flhDC by QseBC and σ 28 (FliA) in enterohaemorrhagic Escherichia coli. Molecular Microbiology, 57(6), 1734–1749. https://doi.org/10.1111/j.1365-2958.2005.04792.x
Clarke, M. B., Hughes, D. T., Zhu, C., Boedeker, E. C., & Sperandio, V. (2006). The QseC sensor kinase: A bacterial adrenergic receptor. Proceedings of the National Academy of Sciences of the United States of America, 103(27), 10420–10425. https://doi.org/10.1073/pnas.0604343103
Hughes, D. T., Clarke, M. B., Yamamoto, K., Rasko, D. A., & Sperandio, V. (2009). The QseC Adrenergic Signaling Cascade in Enterohemorrhagic E. coli (EHEC). PLOS Pathogens, 5(8), e1000553. https://doi.org/10.1371/journal.ppat.1000553
Rooks, M. G., Veiga, P., Reeves, A. Z., Lavoie, S., Yasuda, K., Asano, Y., Yoshihara, K., Michaud, M., Wardwell-Scott, L., Gallini, C. A., Glickman, J. N., Sudo, N., Huttenhower, C., Lesser, C. F., & Garrett, W. S. (2017). QseC inhibition as an antivirulence approach for colitis-associated bacteria. Proceedings of the National Academy of Sciences of the United States of America, 114(1), 142–147. https://doi.org/10.1073/pnas.1612836114
Sperandio, V., Torres, A. G., & Kaper, J. B. (2002). Quorum sensing Escherichia coli regulators B and C (QseBC): A novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E. coli. Molecular Microbiology, 43(3), 809–821. https://doi.org/10.1046/j.1365-2958.2002.02803.x
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