Coding

Part:BBa_K4010003

Designed by: Jessica Wang   Group: iGEM21_McMaster   (2021-10-15)


Colicin E9 with His-tag and RBS

E9 colicin is a bacteriocin produced by certain E. coli to kill closely related strains via cleaving bacterial chromosomal DNA. A separate immunity protein is required to prevent its DNAse activity in the host cell. The E9 colicin is tagged with a 10x his tag at the C-terminal for purification and quantification purposes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None