Regulatory

Part:BBa_K4005001

Designed by: Ke ZHU   Group: iGEM21_Shanghai_Metro_Utd   (2021-10-12)


promoter

promoter

Profile

Name: P7

Base Pairs: 19bp

Origin: T7 phage, genome

Properties: A promoter for initiation of the transcription.

Construct design

Figure 1. The rANG protein expression box..

The profiles of every basic part are as follows:

Proof of function

We did SDS-PAGE assey of protein and have obtained Freeze-dried powder of the ANG protein produced by our engineered Escherichia coli (Figure 2).

Figure 2a. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..
Figure 2b. SDS-PAGE assey of protein and Freeze-dried powder of the ANG protein..

Reference

1.World Health Organization Estimated Deaths 2012.http://www.who.int/entity/healthinfo/global_burden_disease/GHE_Deaths_2012_country.xls?ua=1

2.Biography.com Editors. (2017, April 28). Muhammad Ali. Biography. https://www.biography.com/athlete/muhammad-ali

3.Parkinson disease: MedlinePlus genetics. (n.d.). MedlinePlus-Health Information from the National Library of Medicine.https://medlineplus.gov/genetics/condition/parkinson-disease/

4.Zhang J, et al. Nucleic Acids Res. 2002 Mar 1;30(5):1169-75

5.https://www.ncbi.nlm.nih.gov/Structure/pdb/5EOP

6.Sheng J, et al. Acta Biochim Biophys Sin (Shanghai). 2016 May;48(5):399-410. doi: 10.1093/abbs/gmv131.

7.Aparicio-Erriu IM, et al. Front Neurosci. 2012 Nov 19;6:167. doi: 10.3389/fnins.2012.00167.

8.Wang YN, et al. Cancer Cell. 2018 Apr 9;33(4):752-769.e8. doi: 10.1016/j.ccell.2018.02.012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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