Plasmid_Backbone

Part:BBa_K3963003

Designed by: Silja Malkewitz   Group: iGEM21_Heidelberg   (2021-10-19)


pBAV1k-lacI-Trc

pBAV1k-lacI-Trc plasmid backbone derived from the pBWB162 which is designed by Keith Tyo [1] and is based on the pBAV1k origin of replication. This plasmid backbone contains an ligation side with PvuI and NcoI behind a trc promoter, lacI behind lacIq promoter and a kanamycin selection marker.

Reference

[1] Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1. Biggs BW, Bedore SR, Arvay E, Huang S, Subramanian H, McIntyre EA, Duscent-Maitland CV, Neidle EL, Tyo KEJ. Nucleic Acids Res. 2020 Apr 4. pii: 5815822. doi: 10.1093/nar/gkaa167. 10.1093/nar/gkaa167 PubMed 32246719

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4297
    Illegal NheI site found at 2807
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4303
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4297
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4297
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4297
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4312
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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Categories
Parameters
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