Inducible expression of antitoxin MazE by pBAD
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BamHI site found at 1144
Illegal BamHI site found at 1283
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
- 1000Illegal SapI site found at 961
pBAD has been intensively investigated by previous iGEM projects and has tight regulation upon downstream genes. It is a suitable promoter for regulating antitoxin genes because pBAD can induce very high levels of MazE expression to neutralize toxic effects of MazE. Codon optimization was performed for the expression of the gene in E. coli.
All sequences of subparts are from iGEM registry.