Generator

Part:BBa_K3962352:Design

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-13)


Inducible expression of antitoxin CcdA by pBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

pBAD has been intensively investigated by previous iGEM projects and has tight regulation upon downstream genes. It is a suitable promoter for regulating antitoxin genes because pBAD can induce very high levels of CcdA expression to neutralize toxic effects of CcdB. Codon optimization was performed for the expression of the gene in E. coli.



Source

Source: All sequences of subparts are from iGEM registry.


References