arabinose-mediated inducible expression of toxin CcdB
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BamHI site found at 1144
Illegal BamHI site found at 1902
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
- 1000Illegal BsaI.rc site found at 1352
Illegal SapI site found at 961
pBAD has been intensively investigated by previous iGEM projects and has tight regulation upon downstream genes. It is the most suitable promoter for regulating toxin genes and preventing their toxic effects because glucose can be used to repress its activity. Codon optimization was performed for the expression of the gene in E. coli.
All parts come from the iGEM registry.