arabinose-mediated inducible expression of toxin Hok
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
- 21Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
- 1000Illegal SapI site found at 961
pBAD has been intensively investigated by previous iGEM projects and has tight regulation upon downstream genes. It is the most suitable promoter for regulating toxin genes and preventing their toxic effects because glucose can be used to repress its activity. Codon optimization was performed for the expression of the gene in E. coli.
Hok/Sok is a type I toxin-antitoxin system originating from E. coli. All parts contained in this biobrick come from the iGEM registry.