Coding

Part:BBa_K3957002

Designed by: Derek Johnson   Group: iGEM21_Northern_BC   (2021-09-11)


This part encodes for a human 1, 25 alpha hydroxylase protein

This part consists solely of the coding region for human 1, 25-alpha-hydroxylase protein. This protein functions to catalyze the addition of a hydroxyl group to the 1-C position of 25-hydroxyvitamin D, converting it to the biologically active form of vitamin D (1,25-dihydroxyvitamin D). This enzyme is useful for studying vitamin D in a from a biological environment, as the inactive form (25-hydroxyvitamin D) is found in abundance relative to the active form. To be able to use this part, the binding protein that is naturally bound to inactive vitamin D must be removed. This is typically done by subjecting it to ethanol or DMSO. Once the binding protein is removed, the 1,25-alpha-hydroxylase is able to bind the inactive vitamin D and the reaction may occur.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None