- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1516
- 21Illegal BglII site found at 447
Illegal BglII site found at 1487
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI site found at 531
Illegal SapI.rc site found at 1148
Illegal SapI site found at 845
Do not use KpnI and NheI restriction enzymes as they will cleave the coding sequence.
Gene synthesis from Twist Biosciences.
K, Toda et al. 2017. “Clinical Role of ASCT2 (SLC1A5) in KRAS-Mutated Colorectal Cancer.” International journal of molecular sciences 18(8). https://pubmed.ncbi.nlm.nih.gov/28749408/
Esnault, Cécile et al. 2008. “A Placenta-Specific Receptor for the Fusogenic, Endogenous Retrovirus-Derived, Human Syncytin-2.” Proceedings of the National Academy of Sciences 105(45): 17532–37. https://www.pnas.org/content/early/2008/11/05/0807413105