Coding

Part:BBa_K3942000:Design

Designed by: Igor Blatov   Group: iGEM21_MIPT_MSU   (2021-10-12)


ERVW-1 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1516
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 447
    Illegal BglII site found at 1487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 531
    Illegal SapI.rc site found at 1148
    Illegal SapI site found at 845


Design Notes

Do not use KpnI and NheI restriction enzymes as they will cleave the coding sequence.


Source

Gene synthesis from Twist Biosciences.

References

K, Toda et al. 2017. “Clinical Role of ASCT2 (SLC1A5) in KRAS-Mutated Colorectal Cancer.” International journal of molecular sciences 18(8). https://pubmed.ncbi.nlm.nih.gov/28749408/

Esnault, Cécile et al. 2008. “A Placenta-Specific Receptor for the Fusogenic, Endogenous Retrovirus-Derived, Human Syncytin-2.” Proceedings of the National Academy of Sciences 105(45): 17532–37. https://www.pnas.org/content/early/2008/11/05/0807413105