Plasmid

Part:BBa_K3939235

Designed by: Yu Huang   Group: iGEM21_Tianjin   (2021-10-01)


Carotene-loxp

This is a plasmid that expresses carotene. We use it to indicate whether the systems are all metabolically capable. It is strictly regulated by the tetR-tetO regulatory system. Meanwhile, carrot plasmids without loxp sequence will not be cut by the Cre in the system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2034
    Illegal EcoRI site found at 6253
    Illegal XbaI site found at 2512
    Illegal XbaI site found at 9047
    Illegal XbaI site found at 11437
    Illegal PstI site found at 389
    Illegal PstI site found at 7174
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2034
    Illegal EcoRI site found at 6253
    Illegal NheI site found at 4601
    Illegal NheI site found at 5506
    Illegal PstI site found at 389
    Illegal PstI site found at 7174
    Illegal NotI site found at 13212
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2034
    Illegal EcoRI site found at 6253
    Illegal BglII site found at 4731
    Illegal BglII site found at 5656
    Illegal BglII site found at 7415
    Illegal BglII site found at 12063
    Illegal XhoI site found at 3758
    Illegal XhoI site found at 4430
    Illegal XhoI site found at 4562
    Illegal XhoI site found at 5381
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2034
    Illegal EcoRI site found at 6253
    Illegal XbaI site found at 2512
    Illegal XbaI site found at 9047
    Illegal XbaI site found at 11437
    Illegal PstI site found at 389
    Illegal PstI site found at 7174
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2034
    Illegal EcoRI site found at 6253
    Illegal XbaI site found at 2512
    Illegal XbaI site found at 9047
    Illegal XbaI site found at 11437
    Illegal PstI site found at 389
    Illegal PstI site found at 7174
    Illegal NgoMIV site found at 1670
    Illegal NgoMIV site found at 3006
    Illegal NgoMIV site found at 11292
    Illegal AgeI site found at 2001
    Illegal AgeI site found at 10995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2263
    Illegal SapI.rc site found at 926
    Illegal SapI.rc site found at 7110
    Illegal SapI site found at 13505
    Illegal SapI site found at 15609


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