Plasmid

Part:BBa_K3939233:Design

Designed by: Yu Huang   Group: iGEM21_Tianjin   (2021-10-01)


Carotene regulated by the tetR-tetO regulatory system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2080
    Illegal EcoRI site found at 6299
    Illegal XbaI site found at 2558
    Illegal XbaI site found at 9093
    Illegal XbaI site found at 11483
    Illegal PstI site found at 389
    Illegal PstI site found at 7220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2080
    Illegal EcoRI site found at 6299
    Illegal NheI site found at 4647
    Illegal NheI site found at 5552
    Illegal PstI site found at 389
    Illegal PstI site found at 7220
    Illegal NotI site found at 13258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2080
    Illegal EcoRI site found at 6299
    Illegal BglII site found at 4777
    Illegal BglII site found at 5702
    Illegal BglII site found at 7461
    Illegal BglII site found at 12109
    Illegal XhoI site found at 2013
    Illegal XhoI site found at 3804
    Illegal XhoI site found at 4476
    Illegal XhoI site found at 4608
    Illegal XhoI site found at 5427
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2080
    Illegal EcoRI site found at 6299
    Illegal XbaI site found at 2558
    Illegal XbaI site found at 9093
    Illegal XbaI site found at 11483
    Illegal PstI site found at 389
    Illegal PstI site found at 7220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2080
    Illegal EcoRI site found at 6299
    Illegal XbaI site found at 2558
    Illegal XbaI site found at 9093
    Illegal XbaI site found at 11483
    Illegal PstI site found at 389
    Illegal PstI site found at 7220
    Illegal NgoMIV site found at 1670
    Illegal NgoMIV site found at 3052
    Illegal NgoMIV site found at 11338
    Illegal AgeI site found at 11041
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2309
    Illegal SapI.rc site found at 926
    Illegal SapI.rc site found at 7156
    Illegal SapI site found at 13551
    Illegal SapI site found at 15655


Design Notes

The accuracy of the tetR-tetO regulation system may not be 100%.


Source

From the lab.

References