Composite

Part:BBa_K3939222:Design

Designed by: Yu Huang   Group: iGEM21_Tianjin   (2021-10-01)


GFP with homologous arms


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2422
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2422
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 524
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2422
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2422
    Illegal AgeI site found at 1378
    Illegal AgeI site found at 1947
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 615


Design Notes

The promoter is not strong enough, and GFP expression is not enough.


Source

From the lab.

References