
Composite
Part:BBa_K3939222
Designed by: Yu Huang Group: iGEM21_Tianjin (2021-10-01)
GFP with homologous arms
This is a sequence in which GFP expression is controlled by a consistently expressed promoter. It is integrated into the chromosome and used to indicate whether the eukaryotic cell's chromosome has been cutted.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2422
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2422
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 524
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2422
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2422
Illegal AgeI site found at 1378
Illegal AgeI site found at 1947 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 615
[edit]
Categories
Parameters
None |