Composite

Part:BBa_K3939021:Design

Designed by: Jiajun Ma   Group: iGEM21_Tianjin   (2021-10-01)


Fast degrading GFP with homologous arms


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2974
    Illegal BamHI site found at 2269
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
    Illegal AgeI site found at 2017
    Illegal AgeI site found at 2458
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mutation


Source

From the lab.

References