Composite

Part:BBa_K3939021

Designed by: Jiajun Ma   Group: iGEM21_Tianjin   (2021-10-01)


Fast degrading GFP with homologous arms

This is a sequence in which the fast degrading GFP expression is controlled by a consistently expressed promoter. It is integrated into the chromosome and used to indicate whether the eukaryotic cell's chromosome has been cutted.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2974
    Illegal BamHI site found at 2269
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2974
    Illegal PstI site found at 45
    Illegal PstI site found at 140
    Illegal PstI site found at 500
    Illegal AgeI site found at 2017
    Illegal AgeI site found at 2458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None