
Composite
Part:BBa_K3939021
Designed by: Jiajun Ma Group: iGEM21_Tianjin (2021-10-01)
Fast degrading GFP with homologous arms
This is a sequence in which the fast degrading GFP expression is controlled by a consistently expressed promoter. It is integrated into the chromosome and used to indicate whether the eukaryotic cell's chromosome has been cutted.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2974
Illegal PstI site found at 45
Illegal PstI site found at 140
Illegal PstI site found at 500 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2974
Illegal PstI site found at 45
Illegal PstI site found at 140
Illegal PstI site found at 500 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2974
Illegal BamHI site found at 2269 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2974
Illegal PstI site found at 45
Illegal PstI site found at 140
Illegal PstI site found at 500 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2974
Illegal PstI site found at 45
Illegal PstI site found at 140
Illegal PstI site found at 500
Illegal AgeI site found at 2017
Illegal AgeI site found at 2458 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |