Coding

Part:BBa_K3939001:Design

Designed by: Lingwei Ding   Group: iGEM21_Tianjin   (2021-09-30)


Cas9 with NLS label


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 1188
    Illegal BamHI site found at 2006
    Illegal XhoI site found at 3544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2740
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

if you want to use CRISPR/Cas system to engineer genome of eukaryotic cell, you need to add NLS label on your cas9 protein. This NLS is designed for Saccharomyces cerevisiae.


Source

from lab

References