Coding

Part:BBa_K3939001

Designed by: Lingwei Ding   Group: iGEM21_Tianjin   (2021-09-30)


Cas9 with NLS label

Nuclear localization signal is an amino acid sequence that exists in many proteins, which mediates the translocation of proteins from cytoplasm to nucleus. There are diverse sequences of NLS, and their mechanisms of action are also different. Tianjin_2021 aims to use CRISPR/Cas9 system to make eukaryotic chromosome-free cells, so we design this part to help Cas9 protein enter nucleus and make double strand breaks(DSBs) on chromosomes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 1188
    Illegal BamHI site found at 2006
    Illegal XhoI site found at 3544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2740
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None