
Coding
Part:BBa_K3939001
Designed by: Lingwei Ding Group: iGEM21_Tianjin (2021-09-30)
Cas9 with NLS label
Nuclear localization signal is an amino acid sequence that exists in many proteins, which mediates the translocation of proteins from cytoplasm to nucleus. There are diverse sequences of NLS, and their mechanisms of action are also different. Tianjin_2021 aims to use CRISPR/Cas9 system to make eukaryotic chromosome-free cells, so we design this part to help Cas9 protein enter nucleus and make double strand breaks(DSBs) on chromosomes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 1188
Illegal BamHI site found at 2006
Illegal XhoI site found at 3544 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2740
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |