Composite

Part:BBa_K3933016

Designed by: Marie Guillaume   Group: iGEM21_Ionis_Paris   (2021-10-20)


Ldh regulated by miRNA-141

This part was designed as part of a collaboration between teams IONIS Paris and U Paris BME. It is a reporter system for their detection of a miRNA (BBa_K3878009), developed on the same principle than the Cobalamin riboswitch reporting system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 105
    Illegal SpeI site found at 141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 264
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 105
    Illegal SpeI site found at 141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 105
    Illegal SpeI site found at 141
    Illegal AgeI site found at 723
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 453


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Categories
Parameters
None