Composite

Part:BBa_K3933013

Designed by: Marie Guillaume   Group: iGEM21_Ionis_Paris   (2021-10-20)


Ldh regulated by a magnesium riboswitch

This part was created for the integrated human practice part of the Ionis Paris module. It was realized by adding the riboswitch to the sensing design used for our Cobatect project. Unfortunately, this part couldn't be tested in the lab.

More details can be found on (BBa_K911001) , but we tried to sum up some information found on this part :

When magnesium is not linked to the RNA, the riboswitch works as a regulatory element, truncating transcripts. It has four Mg2+ binding sites, which gives it switch-like functionality. The binding of Mg2+ to these sites causes the regulatory region of the riboswitch to compress, which acts as an anti-terminator in its unbound state. When this anti-terminator action is lost, the gene's transcription is terminated by a downstream terminator (contained in the sequence).

BBa K3933013 schema.png

Thanks to Biorender

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 495
    Illegal AgeI site found at 1995
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1725


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