Composite

Part:BBa_K3933011

Designed by: Marie Guillaume   Group: iGEM21_Ionis_Paris   (2021-10-20)


Ldh regulated by a vitamin B12 riboswitch, optimized version


This part is an improved version of the biobrick BBa_K3933006, as both RBS have been replaced by RBS designed on Salis Lab which are supposed to optimise the expression of the coding sequence for which its designed. More details on BBa_K3933007 .

The Tet R RBS was replaced by the part BBa_K3933008 .

The Lactate Dehydrogenase RBS was remplaced by the part BBa_K3933009 .

Unfortunately, due to lack of time, this part has not been tested in the lab.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1734
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1464


[edit]
Categories
Parameters
None