Ldh regulated by a vitamin B12 riboswitch, optimized version
This part is an improved version of the biobrick BBa_K3933006, as both RBS have been replaced by RBS designed on Salis Lab which are supposed to optimise the expression of the coding sequence for which its designed. More details on BBa_K3933007 .
The Tet R RBS was replaced by the part BBa_K3933008 .
The Lactate Dehydrogenase RBS was remplaced by the part BBa_K3933009 .
Unfortunately, due to lack of time, this part has not been tested in the lab.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 7
Illegal NheI site found at 30
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 1734
- 1000Illegal SapI.rc site found at 1464