Composite

Part:BBa_K3933007:Design

Designed by: Marie Guillaume   Group: iGEM21_Ionis_Paris   (2021-10-19)


Sensing system of Vitamin B12 coupled with Ldh


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1450


Design Notes

For this design, we added spacers between the different basic parts of the construction, to ensure that our design will work as wanted.


Source

This part is a combination between our sensing part with a RFP, and the part with an Ldh induced by an arabinose promoter.

References