Composite

Part:BBa_K3900054

Designed by: Louisa Lehmann   Group: iGEM21_Bielefeld-CeBiTec   (2021-09-30)


pBad:Trz:dTer:BTCA:araBAD:PrompC:GFP:T1

signalling cascade for BCTA detection (bacteria)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2190
    Illegal PstI site found at 2406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal PstI site found at 2190
    Illegal PstI site found at 2406
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 3765
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2190
    Illegal PstI site found at 2406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2190
    Illegal PstI site found at 2406
    Illegal NgoMIV site found at 2285
    Illegal AgeI site found at 495
    Illegal AgeI site found at 3600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4582
    Illegal SapI site found at 3582


[edit]
Categories
Parameters
None