In the AHL mediated quorum sensing system,after reaching the threshold, AHL can combine with endogenous LuxR to form a complex and activate the corresponding promoter PluxR. In this project, we selected the improved promoter Plux_HS of iGEM2018 Tsinghua.The promoter has the advantage of low leakage.After Plux_ HS is activated, the expressed AHL will combine with endogenous LuxR to form a complex and furtherly activate Plux_ HS promoter, so as to achieve the effect of cascade amplification, and finally express a large number of EGFP, making the detection effect significant.
We first verify the circuits by artificially adding AHL.
We want to know the concentration of AHL and set a series of gradients. The results are as follows.
Fig. The fluorescence intensity of engineered bacteria under different concentrations of AHL.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BglII site found at 721
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000Illegal BsaI.rc site found at 1400