RNA

Part:BBa_K3893001

Designed by: Carolina Panchana   Group: iGEM21_Ecuador   (2021-10-20)


dsRNA designed for the target gene Velvet

This sequence is part of the Modular Platform for dsRNA production in the proyect AgroBactory 593.

dsRNA designed to silence the Velvet target gene via RNAi mechanism.

Usage and Biology

Once inside the pathogen, this dsRNA is further processed into RNA interference that can regulate the expression of any gene in the genome. Velvet gene is involved in the development of hyphae, the generation and germination of conidia, and the formation of virulence-inducing secondary metabolites. This has been a target gene to apply dsRNA against FOC R1 [1].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] S. B. Ghag, U. K. S. Shekhawat, and T. R. Ganapathi, “Host-induced post-transcriptional hairpin RNA-mediated gene silencing of vital fungal genes confers efficient resistance against Fusarium wilt in banana,” Plant Biotechnol J, vol. 12, no. 5, pp. 541–553, Jun. 2014, doi: 10.1111/pbi.12158.


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