Coding

Part:BBa_K3855500:Design

Designed by: Sebastian Bayer   Group: iGEM21_BOKU-Vienna   (2021-10-06)


Extracellular endo-inulinase inu2 with a Secretion Signal


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 33
    Illegal SpeI site found at 288
    Illegal SpeI site found at 390
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1296
    Illegal SpeI site found at 33
    Illegal SpeI site found at 288
    Illegal SpeI site found at 390
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 33
    Illegal SpeI site found at 288
    Illegal SpeI site found at 390
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 33
    Illegal SpeI site found at 288
    Illegal SpeI site found at 390
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Type 2s conformity


Source

Protein originates from Aspergillus ficuum. Secretion signal from Lactobacillus plantarum


References