Composite

Part:BBa_K3834036

Designed by: HU Feijun   Group: iGEM21_CityU_HK   (2021-10-21)


PamyB_vrcB_TamyB

To highly increase the expression level of the vrcB gene, vrcB was cloned into a pTAex3 vector1 harboring the amyB promoter and terminator by homologous recombination. Gene fragment harboring PamyB_vrcB_TamyBM served as the second composite part for the cytochrome P450 monooxygenase expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source:

BBa_K3834036 can be amplified from pTAex3-vrcB

.
Reference:

1. Fujii, Tsutomu, et al. "Cloning and nucleotide sequence of the ribonuclease T1 gene (rntA) from Aspergillus oryzae and its expression in Saccharomyces cerevisiae and Aspergillus oryzae." Bioscience, Biotechnology, and Biochemistry 59.10 (1995): 1869-1874.

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