Designed by: HU Feijun Group: iGEM21_CityU_HK (2021-10-21)
To highly increase the expression level of the vrcB gene, vrcB was cloned into a pTAex3 vector1 harboring the amyB promoter and terminator by homologous recombination. Gene fragment harboring PamyB_vrcB_TamyBM served as the second composite part for the cytochrome P450 monooxygenase expression.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
BBa_K3834036 can be amplified from pTAex3-vrcB.
1. Fujii, Tsutomu, et al. "Cloning and nucleotide sequence of the ribonuclease T1 gene (rntA) from Aspergillus oryzae and its expression in Saccharomyces cerevisiae and Aspergillus oryzae." Bioscience, Biotechnology, and Biochemistry 59.10 (1995): 1869-1874.